Extracellular membrane vesicles (EVs) from biological fluids have been proposed as a promising source of protein biomarkers 1.Microvesicles …
In the presence of 1 mM Ca 2+, however, PLD addition resulted in vesicle aggregation, fusion, and precipitation, originating from the interaction of Ca 2+ ions with the negatively charged phospholipids formed in the membranes. Vesicle fusion was monitored by using a novel fusion assay system involving vesicles containing entrapped trypsin and ...
Mutations in the glucosylceramidase beta (GBA) gene are strongly associated with neurodegenerative diseases marked by protein aggregation. GBA encodes the lysosomal enzyme glucocerebrosidase, which breaks down glucosylceramide. A common explanation for the link between GBA mutations and protein aggr …
The neuron-to-neuron propagation of misfolded α-synuclein (αSyn) aggregates is thought to be key to the pathogenesis of synucleinopathies. Recent studies have shown that extracellular αSyn aggregates taken up by the endosomal–lysosomal system can rupture the lysosomal vesicular membrane; however, it remains unclear whether lysosomal rupture leads to the transmission …
Interactions of lysozyme with phospholipid vesicles: effects of vesicle biophysical features on protein misfolding and aggregation. Soft Matter 2012, 8 (35), 9115.
Kinetics of calcium-induced fusion of cardiolipin-phosphatidylcholine vesicles: correlation between vesicle aggregation, bilayer destabilization, and fusion. Biochemistry 1985, 24 (17), 4630-4636.
Recurrent problems are linked to the inherent risk of particle aggregation during high-speed centrifugation in standard isolation procedures and storage of highly enriched extracellular vesicle ...
The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested that the DNA ...
The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested that the DNA ...
Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion. Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs ...
Bacteriorhodopsin has been incorporated into large unilamellar lipid vesicles. Its aggregation behaviour was investigated using X-ray diffraction, electron microscopy, circular dichroism and rotational diffusion measurements. ... Ape-vesicles were produced by treating vesicles with hydroxylamine in the presence of light, causing the loss of the ...
Extracellular vesicles (EVs) are a cluster of nano-sized vesicles of different sizes, cargo, and surface markers that are secreted into the extracellular environment through a variety of mechanisms. They carry various components of the cytoplasm and cell membrane that are selectively loaded into these vesicles. They are secreted by all forms of living cells and …
Aggregation of Phospholipid Based Vesicle Using Triblock Polymer - Volume 1 Issue 56 ... The results show that with an increase of polymer to lipid ratios, the aggregation process was facilitated. The nanoparticles were fabricated at the Self-Assembled Functional Nanomaterials (SAFN) lab under the mentorship of Professor Mu-Ping Nieh. The ...
Notably, possible aggregation of vesicles in these compartmentalized systems was not investigated. The current study is devoted to vesicle aggregation induced by dextran and PEG over a wide range of molecular weights and concentrations of the polymers as a function of the temperature and of the size and concentration of the vesicles.
The evolution of unicellular to multicellular life is considered to be an important step in the origin of life, and it is crucial to study the influence of environmental factors on this process through cell models in the laboratory. In this paper, we used giant unilamellar vesicles (GUVs) as a cell …
Cation-induced aggregation of acidic phospholipid vesicles: the role of fatty acid unsaturation and cholesterol. Chemistry and Physics of Lipids 1991, 57 (1), 59-66.
According to current hypotheses, cell-derived vesicles either form by blebbing and shedding of plasma membrane pieces or they are secreted by cells after fusion of …
Self-limiting aggregation of phospholipid vesicles† N. de Lange, F. A. M. Leermakers and J. M. Kleijn * Lipid vesicles are widely used as model systems to study biological membranes. The self-assembly of such vesicles into vesicle pairs provides further opportunity to study interactions between membranes.
Vesicle aggregation experiments using cationic polymers were performed in a similar way to those with biotin and streptavidin: we started with single vesicles and stepwise added linker molecules to induce aggregation. The linker molecule here is the polycation poly-l-lysine (PLL). We continued the addition of PLL until unlimited aggregation was ...
The self-aggregation of dimethyl-di-n-octylammonium chloride, in diluted aqueous solutions, was studied with various experimental and theoretical techniques: zetametry, conductimetry, dimethyl-di-n-octylammonium and chloride-selective electrodes, tensiometry, NMR spectroscopy ((1)H and DOSY), and molecular modeling (PM3 and molecular dynamic).
Traditionally, liquid ethanol is known to enhance the permeability of lipid membranes and causes vesicle aggregation and fusion. However, how the amphiphilic ethanol molecules perturb the lipid vesicles to facilitate their aggregation or fusion has not been addressed at any level of molecular simulations.
α-Synuclein is an intrinsically disordered protein abundant in presynaptic terminals in neurons and in synaptic vesicles. α-Synuclein's interaction with lipid bilayers is important not only for its normal physiological function but also in its pathological aggregation and deposition as Lewy bodies in Parkinson's disease. α-Synuclein binds preferentially to lipids with acidic …
We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affec …
Vesicle aggregation experiments using cationic polymers were performed in a similar way to those with biotin and streptavidin: we started with single vesicles and stepwise added linker molecules to induce aggregation. The linker molecule here is the polycation poly-L-lysine (PLL). We continued the addition of PLL until unlimited aggregation was ...
Extracellular vesicles (EVs) refer to a group of naturally occurring small lipid bilayer particles that are derived from cells and cannot replicate 1,2 including exosomes (EVs of endosome-origin 3 ...
1 Altmetric. Metrics. Methods that enable specific and sensitive quantification of small extracellular vesicles (sEVs) using flow cytometry are still under development. …
Free base porphyrin (PPhe), derivatized with aminosulfonyl groups linked to the aromatic amino acid phenylalanine at the meso-positions, was mixed with DMPC vesicles. The resulting interaction was studied by absorption, steady-state and transient state fluorescence, at different pHs. At pH=2 to pH=9 …
Aggregation of bacillus thuringiensis Cry1A toxins upon binding to target insect larval midgut vesicles Appl Environ Microbiol ... which included primarily helix alpha1. Following incubation of the Cry1Ab or Cry1Ac toxins with vesicles, the preparations were solubilized by relatively mild conditions, and the toxin antigens were analyzed by ...
Extracellular vesicles (EVs) have vital functions and promise diagnostic and therapeutic applications. ... and prevent aggregation needs to be considered when selecting the most appropriate EV ...
Cells produce a wide variety of extracellular vesicles (subdivided into exosomes and microvesicles), which carry a multitude of cargoes, including proteins, lipids and nucleic acids. These ...
Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential biomedical applications. …
The membrane tubules enclose linear aggregates of particles and protrude into the vesicles. The high stability of the particle-filled tubules implies strongly attractive, membrane-mediated interactions between the particles. The tubular structures may provide a new route to encapsulate nanoparticles reversibly in vesicles.
